How to Reconstitute Peptides with Bacteriostatic Water

What you need

• Lyophilised peptide vial, stored refrigerated.
• Bacteriostatic water (0.9 percent benzyl alcohol preserved).
• Sterile insulin or 1ml syringe with fine-gauge needle.
• Alcohol swabs.

Do not use plain sterile water or tap water. Only bacteriostatic water preserves the reconstituted peptide for multi-week use.

Step-by-step

1. Bring the vial to room temperature for 10 minutes. Cold vials can crack.
2. Swab both rubber stoppers with alcohol and let dry.
3. Draw the chosen volume of bacteriostatic water (see math below).
4. Inject the water slowly against the vial wall, not directly onto the powder.
5. Do not shake. Gently swirl until the powder fully dissolves. This can take a minute.
6. Label the vial with reconstitution date and concentration.
7. Refrigerate immediately.

The concentration math

Peptide amount in mg divided by water volume in ml equals concentration in mg per ml. For a 10mg BPC-157 vial reconstituted with 2ml water, you get 5 mg/ml. On a standard U-100 insulin syringe, each unit mark equals 0.01ml, so 10 units contains 0.5mg peptide at that concentration.

Use our dosage calculator to skip the manual math and get an exact insulin-syringe reading.

Storage after reconstitution

• Refrigerate at 2 to 8 degrees C.
• Use within 4 weeks for most peptides.
• Do not freeze reconstituted solution, freezing damages tertiary structure.
• Protect from direct light.

Common mistakes

• Shaking the vial, causes foaming and can denature the peptide.
• Injecting water directly onto the powder cake at high speed.
• Reconstituting with normal sterile water, then storing beyond a day.
• Forgetting to label concentration, guaranteed dosing error later.